Amu Darya shovelnose P. kaufmanni mature female Taking biopsy for PI. Nukus Balyk hatchery 07.05.26

Life-on-earth.ru (popular science project) https://life-on-earth.ru/ https://t.me/lifeonearthru Серия видеоматериалов о разведении большого амударьинского лопатоноса (Pseudoscaphirhynchus kaufmanni) на рыбоводном предприятии "Нукус Балык" группой специалистов - энтузиастов и волонтёров из России и Узбекистана весной 2026 года. Нукус, Каракалпакстан, Узбекистан Видео-5 07.05.2026 Вторая щуповая проба (биопсия) у зрелой самки (смотрите также видео-1, видео-2 и видео-3 из серии) для определения коэффициента поляризации (Kp) икры перед нерестом, чтобы убедиться в готовности икры к оплодотворению. Рыбоводное предприятие Нукус Балык Нукус, Каракалпакстан, Узбекистан Температура воды в бассейнах: + 21,7 oC (нерестовая). Первая щуповая проба была сделана 05.04.2026 при температуре воды в бассейнах + 17,5 oC и показала коэффициент поляризации 0,13-0,14 (к нересту не готова). Видео-1: https://rutube.ru/video/4ac878aa448cd6b35edfbe0bf0643b7c/ Видео-2: https://rutube.ru/video/3a2c0ccac0da291b4150739fceeab900/ Видео-3: https://rutube.ru/video/1511a2dabee2fc2834d0f7b00a8bf3dd/ Коэффициент поляризации - наиболее надёжный и простой в определении показатель зрелости икры и готовности самки к нересту. Он определяется положением ядра в цитоплазме икринки и является соотношением между расстоянием от ядра (зародышевого пузырька) до края анимального полюса икринки (яйцеклетки) - l и общим диаметром икринки (внутри оболочки икринки) - L (Kp = l / L). Наша команда 07.05.2026: Каллыбек Примбетов, Данил Домрачев, Алексей Черняк, Камалатдин Жанабаев, Ансатбай Ильясов. Series of videos on Big Amu Darya shovelnose sturgeon (Pseudoscaphirhynchus kaufmanni) propagation at the Nukus Balyk fish hatchery by a group of volunteers and enthusiasts from Russia and Uzbekistan in Spring 2026. Nukus, Karakalpakstan, Uzbekistan. Video-5 07.05.2026 Taking the second biopsy from a mature female of Big Amu Darya shovelnose sturgeon (Pseudoscaphirhynchus kaufmanni) - small narrow snout (dwarf) form, weight 139 g, to find out the Polarization Index and determine the female's readiness to spawn. microchip implant 900263004-531348 genetic sample # 21 female with mature eggs Result: Polarization Index less than 0,05 (ready to spawn) Water temperature in the pool is + 21,7 oC (spawning temperature) Nukus Balyk fish hatchery Town of Nukus, Karakalpakstan, Uzbekistan 07.05.2026 The Polarization Index (PI) is determined by the position of the egg nucleus in the cytoplasm. It is the simplest and most reliable indicator that a female is ready to spawn (or not ready yet). PI is the ratio of the distance from the egg's nucleus (germinal vesicle) to the edge of the animal pole, divided by the total diameter of the egg. It is calculated by the following formular: PI = B / A where B is the distance from the top of the nucleus to the cell membrane, and A is the total egg diameter without the outer shell. As an egg matures, the nucleus moves closer to the outer edge (the animal pole). A smaller PI means the nucleus has migrated further and the egg is closer to maturity. The eggs in this female were identified while ultrasound scanning on 04.04.2026: https://youtu.be/UsVURXt704o https://youtu.be/h3AsaqMqdoY On 04.04.2026 a team of specialists, enthusiasts and volunteers from Russia and Uzbekistan examined Amu Darya shovelnose sturgeons (Pseudoscaphirhynchu) initial brood stock at the Nukus Balyk fish hatchery, conducted ultrasound scanning to determine sex and sexual maturity, took some morphological measurements, and collected tissue samples for genetic analysis. On 04.04.2026 the ultrasound scanning of the female showed the presence of mature eggs. On 05.04.2026 the first biopsy was taken to find out the Polarization Index (PI) in eggs. According to PI (13-14) the propagation was planned for May 2026. https://youtu.be/Rcr1Q_WpyVU AcuVista VT880a portable water proof ultrasound scanner was used for ultrasound scanning. It was obtained with the help of Global Greengrants Fund. https://www.greengrants.org/ On 07.05.2026 the second biopsy was taken from the female before spawning to determine Polarization Index (PI) in eggs again and to be sure that eggs are ready to be fertilized. In 2020 Nukus Balyk fish hatchery was registered as the Amu Darya shovelnose sturgeons hatchery. It provided the Amu Darya shovelnose sturgeons conservation project with a line of recirculating aquaculture system (RAS). Water in the system is refreshed from the Amu Darya river through Kyzketken canal. Related links: in English: https://life-on-earth.ru/amu-darya-and-syr-darya-shovelnose-sturgeons/amu-darya-shovelnose-sturgeons-conservation-uzbekistan in Russian: https://life-on-earth.ru/amudarinskie-lopatonosy/amudarinskie-lopatonosy-proekt-sokhraneniya-uzbekistan Some info in both English and Russian can be found by scrolling through the channel from the beginning: https://t.me/lifeonearthru Music: the-tides-seclusion